Epigenetic regulation of cardiac myocyte proliferation/cell cycle - from single cells to organ fu...

Employer
KU Leuven
Location
Europe
Posted
August 22 2017
Discipline
Other
Position Type
Full Time
Organization Type
Academia

We are looking for a talented researcher to investigate epigenomic mechanisms controlling cell cycle activity and growth of cardiac myocytes. This project will build on our recently published findings (Thienpont, Aronsen, Robinson et al., JCI 2017) and contribute to the development of new strategies for enhancing cardiac regeneration and ameliorate disease. Building on our recently published findings (Thienpont, Aronsen, Robinson et al., JCI 2017), we are analysing the contribution of the epigenome in determining and maintaining the post-mitotic adult cardiac myocyte phenotype and how it can be manipulated to enhance regeneration and ameliorate disease. Given that the absence of proliferation is the Achilles' heel of the adult myocyte, underlying the inadequate proliferation required to repair the damaged heart, identifying how cardiac myocyte fate can be reprogrammed is an exciting and clinically relevant area of research. Moreover, the absence of proliferation in the adult heart provides an ideal substrate to examine epigenetic mechanisms independent of cell cycle.


The aim of this project is to probe the role of histone methylation in the regulation ofc ardiac proliferation and in preventing/reversing deleterious hypertrophicremodelling associated with disease and ageing. This research will test the notion that manipulation of histone methylation can be employed as a tool to reprogram cardiac myocyte phenotype, and excitingly enhance proliferation required for repair.


Genomic/epigenomic analysis is performed on cardiac myocytes isolated from genetically modifiedmice (cardiac specific knockout and transgenics, adeno-associated virustransduced), mouse models of disease (aortic banding, myocardial infarction), human tissue and iPS-derived cardiac myocytes. We analyse transcriptomes andepigenomes, including methylomes of cell populations and single cells (next generation sequencing - ChIP-Seq/RNA-Seq/BS-Seq). As evidenced by our publications, we collaborate extensively within KU Leuven and outside to enhance our research.


If you are a talented scientist, with a special interest in understanding transcriptional regulation, including by the epigenome, in regulating cell fate transitions, please apply. It is also important that you wish to dig deeper than the obvious and perform important research that is relevant to human disease mechanisms.


Selected references:


Thienpont, B., J.M. Aronsen, E.L. Robinson, H. Okkenhaug, E. Loche,A. Ferrini, P. Brien, K. Alkass, A. Tomasso, A. Agrawal, O. Bergmann, I.Sjaastad, W. Reik, and H.L. Roderick. 2017. The H3K9 dimethyltransferases EHMT1/2 protect against pathological cardiac hypertrophy. J Clin Invest.127:335-348. doi:10.1172/JCI88353.


Drawnel, F.M.,D. Wachten, J.D. Molkentin, M. Maillet, J.M. Aronsen, F. Swift, I. Sjaastad, N.Liu, D. Catalucci, K. Mikoshiba, C. Hisatsune, H. Okkenhaug, S.R. Andrews, M.D.Bootman, and H.L. Roderick. 2012. Mutual antagonism between IP(3)RII and mi... For more information see https://icts.kuleuven.be/apps/jobsite/vacatures/54282344



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