Postdoctoral Fellow - CRISPR/Cas9 identification of causal vari
Salary £31,115 to £39,004 plus excellent benefits.
Fixed term for 3 years.
A postdoctoral position is available in Cellular Genetics providing an exciting opportunity to use human induced pluripotent stem cell (hiPSC) models to identify causal variants involved in neurodegenerative disease. The positions will be based at the Wellcome Trust Sanger Institute (WTSI) under the leadership of Dr Daniel Gaffney and Dr Andrew Bassett.
The project will form part of an Open Targets (https://www.opentargets.org/) collaboration between the Sanger Institute, GlaxoSmithKline (GSK) and Biogen. The project combines the expertise of the Gaffney (WTSI), Barrett (WTSI) and Johnson (GSK) groups in the analyses of large-scale genome wide association studies (GWAS) and genome-wide expression analysis (GWEA) datasets to identify loci implicated in Alzheimer's and Parkinson's diseases. The applicant will have the opportunity to develop novel CRISPR-based experimental strategies to screen for and identify the causative lesions from this set of candidates, and understand their effects on gene expression networks and other cellular phenotypes.
Candidates with a PhD in a relevant Biological discipline with a strong background in genome engineering, human iPSC culture or in vitro differentiation are strongly encouraged to apply. Excellent cell culture and molecular biology experience, a strong track record of publication and an ability to work both independently and as part of a team are essential. The post-holder will work closely with both the human gene editing (Bassett) and genomics of gene regulation (Gaffney) groups at WTSI and also with the Johnson (GSK) and Estrada (Biogen) groups externally.
- PhD in a relevant subject area (Genetics, Stem Cell Biology, Molecular Biology) or a similar subject
- Strong track record of publications
- Extensive proven experience of mammalian cell culture
- In depth knowledge of genome engineering techniques (e.g. CRISPR, TALEN) and stem cell biology
- Significant experience in molecular biology techniques
- Ability to work independently on projects to the point of publication, as well as part of a team
- Excellent written and verbal communication skills
- Ability to work to tight deadlines
- Ability to test and implement new assays and techniques including their troubleshooting
- Practical experience with human iPS or ES cells
- Experience with in vitro differentiation of mouse or human stem cells
- Practical experience with genome engineering techniques (e.g. CRISPR, TALEN, ZFN)
- Experience with generation and analysis of genomics datasets (e.g. RNA-seq, ATAC-seq, ChIP-seq)
- Experience of advanced cloning techniques (e.g. recombineering, golden gate, Gibson assembly, etc.)
Open Targets is a recently established pioneering public-private initiative between GSK, Biogen, EMBL-EBI (European Bioinformatics Institute) and the WTSI, located on the Wellcome Trust Genome Campus in Hinxton, near Cambridge, UK. Open Targets aims to generate evidence on the biological validity of therapeutic targets and provide an initial assessment of the likely effectiveness of pharmacological intervention on these targets, using genome-scale experiments and analysis. Open Targets aims to provide an R&D framework that applies to all aspects of human disease, and to share its data openly with the scientific community.
The Sanger Institute is a charitably funded research centre focused on understanding the role of genetics in health and disease. We use state of the art large-scale genomic approaches to drive world-leading projects to uncover the basis of genetic and infectious disease. Our goal is to provide results that can be translated into diagnostics, treatments or therapies that reduce global health burdens.
Wellcome Trust Sanger Institute welcomes applications from all candidates irrespective of age, disability, gender, gender identity, sexual orientation, race, religion or belief, or marital or civil partnership status.
Please include a covering letter and CV with your application.