PhD Position in Molecular Biology - DNA demethylation and development

International PhD Programme “Dynamics of Gene Regulation, Epigenetics and DNA Damage Response”
September 22 2016
Position Type
Full Time
During development, DNA demethylation plays an important role in the context of two major concepts: cellular reprogramming and differentiation. First, DNA demethylation is required to reprogram the epigenetic signature to pave the way for pluripotency in the zygote and the primordial germ cells. Second, DNA demethylation of key differentiation factors is a prerequisite for efficient differentiation. There are indications that Gadd45 proteins may be involved in both processes.

We will comprehensively study the role of Gadd45 mediated DNA demethylation using embryonic stem cells as model system combining genome-wide expression and methylation analysis. To this aim, we will characterize ES cells lacking Gadd45 proteins. We will differentiate ES cells in vitro into different lineages and monitor how loss of Gadd45 affects differentiation. Global expression profiling by microarrays and/or RNA-sequencing in combination with global methylation profiling using MeDIP and Gadd45 ChIP-Seq will lead to the identification of direct Gadd45 target genes in this differentiation process. These newly identified target genes can be used for further molecular analysis of Gadd45 mediated DNA demethylation.

We offer

• The possibility to work on a cutting-edge project using state-of-the-art technology in a highly motivated research team

• A stimulating, diverse and international research environment

• Advanced training opportunities

• A fully funded PhD position

Required qualifications

• Master or Diploma

• Motivation to solve complex biological problems

• Excellent communication skills

This job comes from a partnership with Science Magazine and Euraxess

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