Postdoctoral position - gene regulation in hematopoietic differentiation and role of extracellula...

Employer
Charles University in Prague, First Faculty of Medicine,
Location
Czech Republic
Posted
August 22 2016
Discipline
Other
Position Type
Full Time
The available projects:



1. Transcriptional and post-transcriptional regulation during hematopoietic differentiation. This project includes the study of mutual regulation and cooperation of transcription factors and ncRNAs, analysis of transcription factor binding, chromatin structure and modifications, identification of enhancer sequences, enhancer RNAs and 3D looping, differentiation assays etc. The work would also involve some in silico approaches for studying of gene regulatory sequences using own and publicly available whole genome data.



2. Role of extracellular microRNA in tumor biogenesis, dissemination and their usage in tumor diagnostics and relapse prediction.

MicroRNAs are short non-coding RNAs (19-23nt) that negatively regulate gene expression by translational repression and/or mRNA degradation. Extracellular (circulating, secreted) microRNAs are an extracellular form of microRNAs which is in contrast to the longer species of RNA surprisingly stable and detectable in virtually all body fluids. It is well documented that tumor-associated microRNAs are released from the tumor into the circulation. However, the biological function of circulating microRNAs in tumor biogenesis, reprogramming gene expression within target cells or intracellular communication remaining largely enigmatic.

In this project is addressed the question of potential function of extracellular microRNAs in tumor biogenesis by in vivo and in vitro assays on a mouse model and human hematological malignancies. In addition, the utilization of circulating microRNA as tumor biomarkers is examined.



Methods: Cell culturing and differentiation, molecular cloning and mutagenesis, gene manipulations, cell transfections, reporter assays, chromatin immunoprecipitation, chromosome conformation capture (3C, Hi-C), RT-qPCR, FACS, WB, si/shRNAs, enhancer RNA, nucleic acids isolation and quantification, sequencing, immunoassays and fluorescence microscopy, xenotransplantation.



We seek for a motivated candidate (only foreign, PhD degree up to 10 years) capable of innovative thinking and independent work. Candidate should be familiar with cell and molecular biology methods. Bioinformatic data analysis skills are appreciated.



Duration: 2 years

Start: 1.1. 2017

Funding: 2300 Eur / month



Application should be send to Dr. Vitek Pospisil (vitek_pos@hotmail.com, T: +420 732 953372) and should include CV, a cover letter outlining candidate's research interests, a brief summary of current work, and/or laboratory skills.



Application deadline: 28th August 2016



For further details see:

http://patofyziologie.lf1.cuni.cz/microrna-and-transcription-factors

This job comes from a partnership with Science Magazine and Euraxess

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