Postdoctoral Fellow - Radiation and Bone Marrow Transplant - Hui lab
We are looking for a highly motivated and well organized research associate for radiation and bone marrow transplant projects (e.g. total marrow irradiation, image guided targeted therapy) within the Depart of Radiation Oncology (Hui laboratory), Beckman Research Institute, City of Hope. This is a part of the NIH (National Institute of Health) funded research for developing total marrow irradiation program. The successful candidate will participate investigating physical biology and radiation research in oncology though various multi-disciplinary and translational approaches in pre-clinical and clinical studies. With strong support from the department chair (Dr. Jeffrey Wong), one of the main goal is to foster translational and correlative studies to bridge basic science and clinical trials.
Independently conduct multi step experiments (70%):
- Perform animal work including bone marrow transplant, phenotyping with flow cytometry, tissue harvesting, sectioning and staining. The animal studies must be complied with Institutional Animal Care and Use Committee (IACUC) protocols and regulations.
- Perform in vitro cell culture studies
- Confer with Principal Investigator (PI) and other lab members to plan and execute complex experiments; oversee and manage timelines.
- Maintain necessary inventory of supplies and reagents required for experiments.
- Identify and resolve technical problems as they arise.
- Manage day to day laboratory organization
Method development (20%).
- Review literature for relevant procedures or information. Optimize existing procedures and develop new procedures. Evaluate effectiveness of techniques and technologies, assess progress, and present results to PI.
- Accurately document experiments to ensure that work can be replicated by others.
- Collect, analyze, compile, interpret and evaluate research results and report progress to PI.
- Prepare graphical or tabular representation of results for papers and grant proposals.
Minimum Education and Skills Required for Consideration:
- PhD in biology.
- Experience with animal cancer models: leukemia/myeloma, bone metastasis (from breast/prostate cancer),
- Experience performing mouse bone marrow transplant (BMT) and engraftment studies using flow cytometry.
- Demonstrated experience with the following laboratory techniques: sterile technique, cell culture, flow cytometry,
- RNA, DNA isolation and preparation, quantification RT-PCR, elisa assay, western blot.
- Maintaining detailed documentation and writing technical reports.
- Demonstrated experience generating graphs, charts, tables, figures, and graphics for presentation.
- Cell culture: leukemia, myeloma, human and mouse stem cells, colony forming assay, cell cycle, toxicity assay, proliferation assay etc.
- Mouse model: Bone marrow transplant, Leukemia/Myeloma
- Expertise in flow cytometry, quantification RT-PCR, and Western/Southern blotting.
- Radiation (using small irradiator)
Other desired qualifications/interest:
- Total body irradiation and Immune modulation
- Total body irradiation and graft vs host disease (GVHD)
- Bone metastasis and solid tumor
About City of Hope
City of Hope, an innovative biomedical research, treatment and educational institution with over 4000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy. Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research. City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation’s 20 leading cancer centers that develops and institutes standards of care for cancer treatment.
City of Hope is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color, national origin, sex, sexual orientation, gender identity, age, status as a protected veteran, or status as a qualified individual with disability.