Post-doctoral fellow at Massachusetts General Hospital/Harvard Medical School
The Steere/Strle Laboratory at Massachusetts General Hospital does translational studies using samples from patients with Lyme arthritis or rheumatoid arthritis to delineate mechanisms involved in pathogenesis, to identify biomarkers of disease activity, and to develop diagnostic tests for these diseases. This information is used to improve treatment strategies for patients with these disorders.
The projects listed below are often done in parallel in patients with Lyme arthritis or rheumatoid arthritis.
- Identification of HLA-DR-peptide epitopes from microbial or self-proteins in synovial tissue or PBMC and testing of implicated peptides for T and B cell responses.
- Evaluation of implicated T cells for molecular mimicry between homologous microbial antigens and self-proteins by cloning and stimulating cells.
- Assessment of antibody cross-reactivity using recombinant microbial and host proteins and affinity-purified antibodies, and determination of antibody phenotypes and function based on Fc receptor binding, IgG subclass, binding affinity, and glycan profile.
- Characterization of immune cell phenotypes by flow cytometry and single cell RNA-seq.
- Evaluation of host and microbial genetic factors that contribute to dysregulated immunity and adverse patient outcomes.
- Determination of biomarkers, serologic tests, and direct detection assays for improvement of patient diagnosis and treatment.
Many of these projects are done in collaboration with investigators in our Center for Immunology and Inflammatory Diseases, and with those in surrounding institutions, including the Broad Institute, the Ragon Institute, the Forsythe Institute, and the Boston University Biomedical Mass Spectrometry Center.